Oakland/Greifensee: For successful experiments, pipette tips have to be completely inert and must not interfere with samples in any way. To achieve this, being contamination- free is no longer enough. Pipette tips also need to be free of any bioactive components.
Many products on the market are labeled as 100% contamination-free or sterile, but a recent Science publication [1] shows that even these tips can compromise the outcome of scientific work.
In the paper, a scientific team working on a series of molecular biology experiments observed negative results. After checking all possible options they determined that their lack of reliable results were due to specific inhibitors that were associated with the “virgin polypropylene” tip products, purchased from major manufacturers, that were used in the experiments.
Mass spectroscopy analysis of material that washed out from the tubes and tips indicated two products from the manufacturing process that were the likely inhibiting candidates – these were a detergent and a releasing agent (oleamide and DiHEMDA) commonly used by manufacturers of polypropylene accessories.
Independent external analysis of RAININ BioClean tips, using the same method employed in the Science paper to extract and determine the contaminants, showed zero contamination by the two inhibiting molecules.
The absence of bioactive components in the materials, in combination with the proven avoidance of external contaminants (DNA, DNAse, RNAse, ATP, Pyrogens, or PCR Inhibitors) in the production and packaging process, ensures that high-quality RAININ BioClean tips are completely inert and do not influence experimental outcome in any way. For best lab performance and most reliable results.
More information on www.mt.com/rainin
[1] McDonald, G., Hudson, A., Dunn, S., You, H., Baker, G., Whittal, R., Martin, J., Jha, A., Edmondson, D., and A. Holt. 2008. Bioactive Contaminants Leach from Disposable Laboratory Plasticware. Science 332(5903): 917.
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